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Comparison of ethylene carbonate and formamide as components of the hybridization mixture in FISH

Scientia Agricola

Kalinka,Anna; Myśliwy,Monika; Achrem,Magdalena.

ABSTRACT The protocols for in situ hybridization (ISH) techniques can vary considerably; however, they usually include denaturation and hybridization steps. Denaturing compounds are used to reduce denaturation and hybridization temperature, which keeps the proper morphology of the preparation. Formamide is the most commonly used reagent in in situ hybridization to lower the melting temperature. The substitution of toxic formamide for a non-toxic ethylene carbonate at 20 % and 50 % concentration in the hybridization mixture helped obtain a high quality in situ hybridization result with two sequences characteristic for rye, JNK, and Bilby. The results after hybridization, with a duration of 90 min and 16 h, were identical when formamide or ethylene carbonate...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Secale; In situ hybridization; Repeated sequences; Non-toxic.

Ano: 2021

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000401101

Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp MC3B-10

ArchiMer

Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto.

A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its...

Tipo: Text

Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology.

Ano: 2013

URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf